mammocult medium (STEMCELL Technologies Inc)

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STEMCELL Technologies Inc mammocult medium

FIS1 or MFF knockdown suppresses the TIC population in MDA-MB-231 triple negative breast cancer cells. A ) Western blot analysis of the steady-state levels of the DRP1 receptors FIS1, MFF, MID49 and MID51 in parental MDA-MB-231 triple-negative breast cancer (TNBC) cells or those transduced with non-silencing shRNA (MDA-NS). GAPDH served as a loading control. B ) Expression of DRP1 receptors in MDA-MB-231 cells stably transduced with individual shRNAs specific to FIS1, MFF or MID51. C ) The indicated cells were seeded in complete <t>Mammocult</t> medium into 24-well Ultra-Low attachment plates (4 × 10 3 cells per well) and tumorspheres were allowed to propagate for 7 days. The data represent the cumulative analysis of combined counts of tumorsphere cells from a minimum of two independent experiments. In each experiment, analysis of NS, FIS1 and MFF knockdown cells was performed in triplicate, with each triplicate point comprised of tumorspheres pooled from four independent wells ( n ≥ 6). D ) Representative images of tumorspheres from C ). Scale bar: 1000 μm. E , F ) Relative abundance of ALDH-positive cells in the indicated monolayer cultures was identified by flow cytometry using the Aldefluor assay kit. Gating was based on matching negative control cells treated with the specific ALDH inhibitor DEAB according to the manufacturer’s instructions. Representative histograms are shown in E ), and cumulative data are graphed in F ). For FIS1 and MFF knockdown experiments, n = 9 and 3 respectively. Where required, Western blot images were optimized using PowerPoint. For C ) and F ), data are presented as the mean ± SE and were analyzed using a two-tailed Student’s t-test. * denotes p < 0.05

Mammocult Medium, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mammocult+medium/mammocult+medium/pmc12276697-54-16-18
Average 90 stars, based on 1 article reviews

mammocult medium - by Bioz Stars, 2026-09

90/100 stars

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1) Product Images from "The DRP1 receptor FIS1 is critical to the expansion of triple-negative breast cancer tumor-initiating cells"

Article Title: The DRP1 receptor FIS1 is critical to the expansion of triple-negative breast cancer tumor-initiating cells

Journal: Cancer Cell International

doi: 10.1186/s12935-025-03909-5

Figure Legend Snippet: FIS1 or MFF knockdown suppresses the TIC population in MDA-MB-231 triple negative breast cancer cells. A ) Western blot analysis of the steady-state levels of the DRP1 receptors FIS1, MFF, MID49 and MID51 in parental MDA-MB-231 triple-negative breast cancer (TNBC) cells or those transduced with non-silencing shRNA (MDA-NS). GAPDH served as a loading control. B ) Expression of DRP1 receptors in MDA-MB-231 cells stably transduced with individual shRNAs specific to FIS1, MFF or MID51. C ) The indicated cells were seeded in complete Mammocult medium into 24-well Ultra-Low attachment plates (4 × 10 3 cells per well) and tumorspheres were allowed to propagate for 7 days. The data represent the cumulative analysis of combined counts of tumorsphere cells from a minimum of two independent experiments. In each experiment, analysis of NS, FIS1 and MFF knockdown cells was performed in triplicate, with each triplicate point comprised of tumorspheres pooled from four independent wells ( n ≥ 6). D ) Representative images of tumorspheres from C ). Scale bar: 1000 μm. E , F ) Relative abundance of ALDH-positive cells in the indicated monolayer cultures was identified by flow cytometry using the Aldefluor assay kit. Gating was based on matching negative control cells treated with the specific ALDH inhibitor DEAB according to the manufacturer’s instructions. Representative histograms are shown in E ), and cumulative data are graphed in F ). For FIS1 and MFF knockdown experiments, n = 9 and 3 respectively. Where required, Western blot images were optimized using PowerPoint. For C ) and F ), data are presented as the mean ± SE and were analyzed using a two-tailed Student’s t-test. * denotes p < 0.05

Techniques Used: Knockdown, Western Blot, Transduction, shRNA, Control, Expressing, Stable Transfection, Flow Cytometry, Negative Control, Two Tailed Test

Knockdown of FIS1 or MFF in HS-578t TNBC cells leads to a reduction in relative TIC abundance and tumorsphere growth. A ) Western blot analysis of FIS1 and MFF levels in HS-578t TNBC cells after stable transduction with the indicated shRNAs. B , C ) Relative abundance of ALDH-positive cells in the indicated monolayer cultures was identified as in Fig. E. Representative histograms are shown in B ), and data from two independent knockdown experiments for each DRP1 receptor were done in triplicate and are graphed in C ). D - F ) Cells were seed in complete Mammocult medium into 24-well Ultra-Low attachment plates (4 × 10 3 cells per well), and tumorspheres were allowed to propagate for 5 days. D ) Representative images of tumorspheres are shown. Scale bar: 1000 μm. E ) Tumorsphere number and F ) total tumorsphere diameter per well were quantified with Image J software using a previously published method . The data represent the cumulative analysis of three independent experiments. In each experiment, analysis of NS, FIS1 and MFF knockdown tumorspheres was performed as described in Fig. C. Any structures less than 40 μm in diameter were not considered to represent tumorspheres and were excluded from the analysis. Where required, Western blot images were optimized using PowerPoint. For C ), E ), and F ), data are presented as the mean ± SE and were analyzed statistically using a two-tailed Student’s t-test. * denotes p < 0.05

Figure Legend Snippet: Knockdown of FIS1 or MFF in HS-578t TNBC cells leads to a reduction in relative TIC abundance and tumorsphere growth. A ) Western blot analysis of FIS1 and MFF levels in HS-578t TNBC cells after stable transduction with the indicated shRNAs. B , C ) Relative abundance of ALDH-positive cells in the indicated monolayer cultures was identified as in Fig. E. Representative histograms are shown in B ), and data from two independent knockdown experiments for each DRP1 receptor were done in triplicate and are graphed in C ). D - F ) Cells were seed in complete Mammocult medium into 24-well Ultra-Low attachment plates (4 × 10 3 cells per well), and tumorspheres were allowed to propagate for 5 days. D ) Representative images of tumorspheres are shown. Scale bar: 1000 μm. E ) Tumorsphere number and F ) total tumorsphere diameter per well were quantified with Image J software using a previously published method . The data represent the cumulative analysis of three independent experiments. In each experiment, analysis of NS, FIS1 and MFF knockdown tumorspheres was performed as described in Fig. C. Any structures less than 40 μm in diameter were not considered to represent tumorspheres and were excluded from the analysis. Where required, Western blot images were optimized using PowerPoint. For C ), E ), and F ), data are presented as the mean ± SE and were analyzed statistically using a two-tailed Student’s t-test. * denotes p < 0.05

Techniques Used: Knockdown, Western Blot, Transduction, Software, Two Tailed Test

other:

Article Title: Establishment and characterization of patient-derived tongue squamous cell carcinoma cell lines.
Article Snippet: The orosphere assay was performed as detailed in Gawas et al. [19] Briefly, 10,000 cells were grown in ultralow attachment condition in Mammocult medium (Stemcell technologies, Canada) and the spheroids formed were counted.

Article Title: Establishment and characterization of patient-derived tongue squamous cell carcinoma cell lines
Article Snippet: The orosphere assay was performed as detailed in Gawas et al. [ ] Briefly, 10,000 cells were grown in ultralow attachment condition in Mammocult medium (Stemcell technologies, Canada) and the spheroids formed were counted.

Tube Formation Assay:

Article Title: Supporting Information for Pharmacological modulation of RB1 activity mitigates resistance to neoadjuvant chemotherapy in locally advanced rectal cancer
Article Snippet: After 24 hours, cells were treated with different concentrations of drugs and cultured at 37°C for 96 hours, and the number of viable cells was measured by using CellTiter-Glo Luminescent Cell Viability Assay (#G7573, Promega, Madison, WI) according to the manufacturer’s instructions. .. For the tumorsphere formation assay, single-cell suspensions were plated (5000 cells/well) in 12 well ultra-low attachment plates with Mammocult medium (STEMCELL Technologies, Vancouver, BC), supplemented with fresh hydrocortisone (0.5 μg/ml) and heparin (1:500). ..

Single Cell:

Cell Culture:

Article Title: Nanofibrillar Cellulose Hydrogels with Anionic Surface Modifications for Modulating Macrophage Phenotype in 3D Culture
Article Snippet: CD14+ cells were magnetically separated with MS columns (Miltenyi Biotec) according to the manufacturer’s instructions, after which they were immediately used for 2D or 3D culture. .. Cells were cultured in the MammoCult medium (STEMCELL Technologies) supplemented with 10% MammoCult proliferation supplement (STEMCELL Technologies), 2 mM l -glutamine (Thermo Fisher Scientific), 4 μg/mL heparin (Sigma-Aldrich), 20 μg/mL gentamicin (Sigma-Aldrich), 0.1 μg/mL amphotericin B (Biowest), and 1% penicillin/streptomycin (Lonza). ..

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